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The Role of Peptide Growth Factors in Epithelial Ovarian Cancer

From the Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, the Departments of Medicine and Microbiology/Immunology, and the Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina

Abstract

We studied the effect of epidermal growth factor, platelet-derived growth factor, fibroblast growth factor, and transforming growth factor-beta on proliferation of four epithelial ovarian cancer cell lines (OVCA 420, OVCA 429, OVCA 432, and OVCA 433). Epidermal growth factor stimulated growth of OVCA 429 cells (P=.0001) and OVCA 433 cells (P=.0002). Platelet-derived growth factor did not stimulate growth of any of the cell lines. Fibroblast growth factor stimulated growth of OVCA 420 cells (P=.003). Transforming growth factor-beta inhibited growth of OVCA 420 cells (P=.0001), OVCA 432 cells (P=.003), and OVCA 433 cells (P=.004). To detect production of known growth factors by the cancer cell lines, we tested the effect of cancer cell-conditioned media on proliferation of cell lines known to respond to growth factors. Only media exposed to OVCA 433 cells were found to contain activity that mimicked one of the known growth factors (transforming growth factor-beta). These results suggest that individual ovarian cancers vary widely in their response to and production of known peptide growth factors. Finally, we found that OVCA 429- conditioned medium significantly inhibited proliferation of mitogen-stimulated lymphocytes (P<.0001). The characteristics of this immunosuppressive factor were distinct from those of transforming growth factor-beta. Production of this factor by an immortalized cell line provides a unique opportunity to identify an immunosuppressive substance associated with ovarian cancer.