Obstetrics & Gynecology Email Alerts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Obstetrics & Gynecology 1984;64:767-772
© 1984 by The American College of Obstetricians and Gynecologists
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WAGNER, D.
Right arrow Articles by GISSMANN, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WAGNER, D.
Right arrow Articles by GISSMANN, L.

Identification of Human Papillomavirus in Cervical Swabs by Deoxyribonucleic Acid In Situ Hybridization

DIETER WAGNER, MD, HANS IKENBERG, MD, NORBERT BOEHM, MD and LUTZ GISSMANN, PhD

From Cytologisches Laboratorium am Evangelischen Diakoniekrankenhaus, Lehrkrankenhaus der Universitaet Freiburg, and Pathologisches Institut der Universitaet, 7800 Freibrug; and Institut fuer Virusforschung, Deutsches Krebsforschungszentrum, 69 Heidelberf, Federal Republic of Germany.

A series of 47 lesions diagnosed cytologically as cervical intraepithelial neoplasia (CIN) III in 22 cases, CIN I/II in 13 cases, and 12 cases showing abnormal smears consistent with human papillomavirus infection were analyzed. Thirty-six cases with negative cytology were used as the control group. Sixty-eight percent of CIN III were positive for a mixture of human papillomavirus 16 and human papillomavirus 18, 18% reacted with human papillomavirus 6 or 11, and 14% were negative. Of the group with CIN I/II or with abnormal Papanicolaou smears, approximately one-third contained human papillomavirus 6 (11) and one-third human papillomavirus 16 and 18. Only 11% of the samples from the control group hybridized with human papillomavirus 6 (11), the others were negative with either probe. The data obtained by the rapid in situ hybridization of cervical cells are in agreement with the presence of human papillomavirus 16 and 18 in a high proportion of cervical carcinoma and carcinoma in situ lesions. Thus, the method can be applied to test the hypothesis that a lesion containing human papillomavirus 16/18 positive cells has a higher risk of progressing to cancer than a lesion harboring human papillomavirus 6 or 11.




This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
D. Kenny, L.-P. Shen, and J. A. Kolberg
Detection of Viral Infection and Gene Expression in Clinical Tissue Specimens Using Branched DNA (bDNA) In Situ Hybridization
J. Histochem. Cytochem., September 1, 2002; 50(9): 1219 - 1227.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
L. Muderspach, S. Wilczynski, L. Roman, L. Bade, J. Felix, L. A. Small, W. M. Kast, G. Fascio, V. Marty, and J. Weber
A Phase I Trial of a Human Papillomavirus (HPV) Peptide Vaccine for Women with High-Grade Cervical and Vulvar Intraepithelial Neoplasia Who Are HPV 16 Positive
Clin. Cancer Res., September 1, 2000; 6(9): 3406 - 3416.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
C. P. Crum
Detecting Every Genital Papilloma Virus Infection : What Does It Mean?
Am. J. Pathol., December 1, 1998; 153(6): 1667 - 1671.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American College of Obstetricians and Gynecologists.