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Identification of Neoplastic Versus Normal Cells in Human Cervical Cell Culture

From the Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington, Vermont.
Send reprint requests to M. J. Cray, MD
Dept of Obstetrics and Gynecology University of Vermont College of Medicine Burlington, VT 05401

Comparison of hematoporphyrin derivative (Hp-D) localization and subsequent fluorescence in normal and malignant human cervical cell tissue culture preparations was made in an attempt to clarify the specificity of Hp-D for cancer detection. The studies clearly demonstrated that when fluorescence is used as the criterion, malignant cells have a markedly increased affinity for Hp-D. The specificity of Hp-D localization and fluorescent identification of cancer cells was confirmed by using mixed cultures of benign and malignant cells as an experimental model of the clinical problem of cancer detection. The results of these studies support the concept of malignant or potentially malignant cells revealing themselves by Hp-D fluorescence as well as the need for revaluation of traditional morphologic criteria for determining malignancy.