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From the Department of Embryology and Teratology, Tel Aviv University, Medical School, Ch. Sheba Medical Center, Tel-Hashomer, Israel
Abstract
A technic has been developed to isolate syncytiotrophoblast of human term placentas. The technic is a combination of mechanical segregation and chemical dissociation of small fragments of placental villi, followed by isolation through sedimentation and purification by elective transfers in tissue culture. By means of repealed transfers, a cell population was obtained in which 80 to 90% of the viable elements were pure syncytiotrophoblast. The viability of the isolated syncytiotrophoblast was proved. The material seems suitable for detailed investigation of the characteristics of syncytium from term placentas.
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