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Obstetrics & Gynecology 2007;110:1130-1136
© 2007 by The American College of Obstetricians and Gynecologists
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ORIGINAL ARTICLES

Placenta-Derived, Cellular Messenger RNA Expression in the Maternal Blood of Preeclamptic Women

Shiho Okazaki, MD1, Akihiko Sekizawa, MD1, Yuditiya Purwosunu, MD1,2, Antonio Farina, MD1,3, Noroyono Wibowo, MD2 and Takashi Okai, MD1

From the 1Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan; 2Department of Obstetrics and Gynecology, University of Indonesia, Cipto Mangunkusumo National Hospital, Jakarta, Indonesia; and 3Department of Histology and Embryology Division of Prenatal Medicine, University of Bologna, Bologna, Italy.

ABSTRACT

OBJECTIVE: To perform gene expression profiling and real-time quantitative reverse-transcription polymerase chain reaction (PCR) analysis to identify biomarkers of preeclampsia in cellular messenger RNA (mRNA) from maternal blood.

METHODS: We performed a microarray analysis with five maternal blood samples from women with preeclampsia and five matched control subjects. Up-regulated gene expression was further analyzed through reverse-transcription PCR analysis with 28 consecutive blood samples from women affected with preeclampsia and 29 controls.

RESULTS: Both pregnancy-specific ß1 glycoprotein and trophoblast glycoprotein were selected based on microarray analysis. Reverse-transcription PCR analysis detected significantly increased mRNA concentrations among women in the preeclampsia group. When stratified according to mild or severe preeclampsia, 19.2-fold and 41.8-fold increases in pregnancy-specific ß1 glycoprotein and 8.3-fold and 10.6-fold increases in trophoblast glycoprotein were observed, respectively. Among women with hemolysis, elevated liver enzymes, and low platelet count syndrome, 51.6-fold and 13.1-fold increases in pregnancy-specific ß1 glycoprotein and trophoblast glycoprotein were observed, respectively. In the preeclampsia group, pregnancy-specific ß1 glycoprotein correlated with severity of proteinuria (P<.001) and systolic blood pressure (P=.01).

CONCLUSION: The mRNA expression of pregnancy-specific ß1 glycoprotein and trophoblast glycoprotein is up-regulated in cells circulating within blood from women with preeclampsia, and pregnancy-specific ß1 glycoprotein expression is positively correlated with the clinical severity of preeclampsia.

LEVEL OF EVIDENCE: II






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Copyright © 2007 by the American College of Obstetricians and Gynecologists.