Obstetrics & Gynecology Email Alerts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Obstetrics & Gynecology 2002;100:759-764
© 2002 by The American College of Obstetricians and Gynecologists
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Obata-Yasuoka, M.
Right arrow Articles by Hayashi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Obata-Yasuoka, M.
Right arrow Articles by Hayashi, H.

ORIGINAL RESEARCH

A Multiplex Polymerase Chain Reaction–Based Diagnostic Method for Bacterial Vaginosis

Mana Obata-Yasuoka, MD, William Ba-Thein, MD, PhD, Hiromi Hamada, MD, PhD and Hideo Hayashi, MD, PhD

From the Department of Obstetrics and Gynecology, Institute of Clinical Medicine, and Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.

Address reprint requests to: Mana Obata-Yasuoka, MD, Institute of Clinical Medicine, University of Tsukuba, Department of Obstetrics and Gynecology, Tsukuba, 305-8575, Japan; E-mail: md995455{at}md.tsukuba.ac.jp.

OBJECTIVE: To develop a polymerase chain reaction (PCR)-based diagnostic method for bacterial vaginosis using bacterial vaginosis–associated anaerobes.

METHODS: A multiple PCR assay was developed using primers specific to 16S ribosomal deoxyribonucleic acid (DNA) (Mobiluncus mulieris and Mobiluncus curtisii), nanH (Bacteroides fragilis), and an internal spacer region of ribosomal DNA (Gardnerella vaginalis). The vaginal swabs from pregnant and nonpregnant women were examined by Gram stain–based Nugent scoring system. One hundred seventy-two samples of 853 Gram stain–interpretable samples were randomly selected and subjected to multiplex PCR assay.

RESULTS: The sensitivity of the PCR assay ranged from 103 to 104 colony-forming units per vaginal swab. The prevalence of the bacterial vaginosis, intermediate, and normal categories was found by Nugent scoring system to be 21.6% (184/853), 26.0% (222/853), and 52.4% (447/853), respectively. By the multiplex PCR–based diagnostic method, 20.3% (35/172) of the samples were identified as bacterial vaginosis. The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value of multiplex PCR in comparison with Gram stain examination were 78.4% (95% confidence interval [CI] 65.1%, 91.6%), 95.6% (95% CI 92.1%, 99.0%), 82.9% (95% CI 70.4%, 95.4%), and 94.2% (95% CI 90.3%, 98.1%), respectively.

CONCLUSION: This multiplex PCR can be used as a diagnostic or screening test for bacterial vaginosis.




This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
B. E. Sha, H. Y. Chen, Q. J. Wang, M. R. Zariffard, M. H. Cohen, and G. T. Spear
Utility of Amsel Criteria, Nugent Score, and Quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for Diagnosis of Bacterial Vaginosis in Human Immunodeficiency Virus-Infected Women
J. Clin. Microbiol., September 1, 2005; 43(9): 4607 - 4612.
[Abstract] [Full Text] [PDF]

Home page
 JWatch GeneralHome page
Bacterial Vaginosis: Making the Diagnosis and Reducing the Incidence
Journal Watch (General), November 26, 2002; 2002(1126): 2 - 2.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American College of Obstetricians and Gynecologists.